Anticorps Polyclonal de lapin anti-ELL2

• Phare
• Validé par KD/KO

ELL2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 12727-1-AP

Synonymes

ELL 2

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Galerie de données de validation

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  WB analysis using 12727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12727-1-AP (ELL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12727-1-AP (ELL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12727-1-AP

  IP result of anti-ELL2 (IP:12727-1-AP, 4ug; Detection:12727-1-AP 1:300) with mouse brain tissue lysate 3440ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules L02, tissu cérébral de rat, tissu cérébral de souris, tissu d'estomac de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12727-1-AP cible ELL2 dans les applications de WB, IF, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELL2 Protéine recombinante Ag3428
• Nom complet: elongation factor, RNA polymerase II, 2
• Masse moléculaire calculée: 640 aa, 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC028412
• Symbole du gène: ELL2
• Identification du gène (NCBI): 22936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELL2 (Elongation Factor for RNA Polymerase II 2) is a key transcriptional elongation factor that regulates RNA polymerase II (Pol II) during mRNA synthesis (PMID: 34265249). It belongs to the ELL gene family, which includes ELL1, ELL2, and ELL3, and plays a crucial role in controlling the elongation phase of transcription (PMID: 9108030). ELL2 is an androgen-responsive gene that is expressed by prostate epithelial cells and is frequently down-regulated in prostate cancer (PMID: 29179998).

Publications

Species	Application	Title
mouse	WB	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang ; KD Validated
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	ChIP	Cell Death Dis; TRIB1 regulates liver regeneration by antagonizing the NRF2-mediated antioxidant response; Authors - Xinyue Sun
mouse	WB	J Biol Chem; Cytoplasmic poly(A)-binding protein 1 (PABPC1) interacts with the RNA-binding protein hnRNPLL and thereby regulates immunoglobulin secretion in plasma cells.; Authors - Yuanzheng Peng
human	WB	FASEB J; Androgen receptor splicing variant 7 (ARv7) promotes DNA damage response in prostate cancer cells; Authors - Haoge Luo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Beth (Verified Customer) (10-05-2017)

Using LI-COR imaging system.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RWPE1, PC-3

ELL2 Antibody Western Blot validation (1:500 dilution) in RWPE1, PC-3 (Cat no:12727-1-AP)

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