Anticorps Polyclonal de lapin anti-ELMO1

ELMO1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26974-1-PBS

Synonymes

ELMO 1, engulfment and cell motility 1

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Galerie de données de validation

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  WB analysis using 26974-1-AP (same clone as 26974-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26974-1-AP (ELMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 26974-1-AP (same clone as 26974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 26974-1-AP (same clone as 26974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 26974-1-AP (same clone as 26974-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 26974-1-AP (same clone as 26974-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ELMO1 antibody (26974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 26974-1-AP (same clone as 26974-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ELMO1 antibody (26974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26974-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26974-1-PBS cible ELMO1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ELMO1 Protéine recombinante Ag25635
• Nom complet: engulfment and cell motility 1
• Poids moléculaire observé: 80~84 kDa
• Numéro d’acquisition GenBank: BC114341
• Symbole du gène: ELMO1
• Identification du gène (NCBI): 9844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ELMO1 (Engulfment and cell motility protein 1) is a cytoplasmic adapter protein that physically associates with members of the Dock-A family of Rac-GEFs, of which Dock1 and Dock2 are the best characterized (PMID: 24821968). ELMO1 is expressed in platelets and negatively regulates integrin-mediated platelet spreading. Upregulated ELMO1 expression is associated with a poor prognosis in hepatocellular carcinoma (HCC), as well as increased cell growth, invasion, migration, angiogenesis and EMT in vitro and in vivo (PMID: 31324602).

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