Anticorps Polyclonal de lapin anti-ELOVL4

• Phare
• Validé par KD/KO

ELOVL4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 55023-1-AP

Synonymes

STGD2, ELOVL fatty acid elongase 4, ELOVL FA elongase 4, EC:2.3.1.199, ADMD

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Galerie de données de validation

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  WB analysis using 55023-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 55023-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 55023-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55023-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55023-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 55023-1-AP

  Immunofluorescent analysis of A431 cells using 55023-1-AP (ELOVL4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules A431, cellules Y79, tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

55023-1-AP cible ELOVL4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: NM_022726
• Symbole du gène: ELOVL4
• Identification du gène (NCBI): 6785
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ELOVL4, also named as ADMD, STGD2 and STGD3, belongs to the ELO family. It is a component of the fatty acid elongation system. Elovl4 is involved in the biosynthesis of very long chain fatty acids. It seems to represent a photoreceptor-specific component of the fatty acid elongation system residing on the endoplasmic reticulum. ELOVL4 may be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps. Defects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3). The antibody is specific to ELOVL4.

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Unraveling the spatial organization and development of human thymocytes through integration of spatial transcriptomics and single-cell multi-omics profiling; Authors - Yanchuan Li ; KD Validated
human	WB	Ocul Surf; PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC).; Authors - Sun Woong Kim
mouse	IHC	Ocul Surf; Ocular surface alkali injury damages meibomian glands in mice.; Authors - Shin Mizoguchi
mouse	WB	Int J Mol Sci; In Human and Mouse Spino-Cerebellar Tissue, Ataxin-2 Expansion Affects Ceramide-Sphingomyelin Metabolism.; Authors - Nesli-Ece Sen
rat	WB	Invest Ophthalmol Vis Sci; Isotretinoin Impairs the Secretory Function of Meibomian Gland Via the PPARγ Signaling Pathway.; Authors - Peng Zhang
human	WB	Lipids; Phytosphingosine Increases Biosynthesis of Phytoceramide by Uniquely Stimulating the Expression of Dihydroceramide C4-desaturase (DES2) in Cultured Human Keratinocytes.; Authors - Hyun Kyung Choi

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