Anticorps Polyclonal de lapin anti-EMB

EMB Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 13946-1-AP

Synonymes

EMB, Embigin, embigin homolog (mouse)

---

Galerie de données de validation

• 

  WB analysis of HeLa using 13946-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13946-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13946-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of mouse testis using 13946-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13946-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13946-1-AP cible EMB dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EMB Protéine recombinante Ag5031
• Nom complet: embigin homolog (mouse)
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC059398
• Symbole du gène: EMB
• Identification du gène (NCBI): 133418
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycolyated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Boyan (Verified Customer) (10-04-2021)

calculated size 37 kd. This antibody recognized major bands at 65, 55 kd, and a very weak band ~45 kd. All of them were PNGaseF insensitive, suggesting they were not glycosylated form of this protein.

• Applications: Western Blot