Anticorps Polyclonal de lapin anti-EMB

EMB Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13946-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 13946-1-AP (same clone as 13946-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13946-1-AP (same clone as 13946-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 13946-1-AP (same clone as 13946-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 13946-1-AP (same clone as 13946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 13946-1-AP (same clone as 13946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13946-1-PBS cible EMB dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EMB Protéine recombinante Ag5031
• Nom complet: embigin homolog (mouse)
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC059398
• Symbole du gène: EMB
• Identification du gène (NCBI): 133418
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycolyated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)

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