Anticorps Recombinant de lapin anti-Emerin

Emerin Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230089C4

N° de cat : 82888-2-PBS

Synonymes

EMD, 230089C4, EDMD, LEMD5, STA

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Galerie de données de validation

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  IHC staining of human thyroid cancer using 82888-2-RR (same clone as 82888-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (Emerin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 82888-2-RR (same clone as 82888-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82888-2-RR (Emerin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82888-2-RR (same clone as 82888-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-2-RR, Clone: 230089C4 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82888-2-RR (same clone as 82888-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-2-RR, Clone: 230089C4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82888-2-RR (same clone as 82888-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human Emerin (82888-2-RR, Clone:230089C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82888-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82888-2-RR against Human Emerin were performed. The affinity constant is 1.71 nM.

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Informations sur le produit

82888-2-PBS cible Emerin dans les applications de IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Emerin Protéine recombinante Ag0236
• Nom complet: emerin
• Masse moléculaire calculée: 34 kDa
• Numéro d’acquisition GenBank: BC000738
• Symbole du gène: EMD
• Identification du gène (NCBI): 2010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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