Anticorps Recombinant de lapin anti-Emerin

• Phare
• Validé par KD/KO

Emerin Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230089B12

N° de cat : 82888-3-PBS

Synonymes

EMD, 230089B12, EDMD, LEMD5, STA

---

Galerie de données de validation

• 

  WB analysis using 82888-3-RR (same clone as 82888-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82888-3-RR (Emerin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

  WB result of Emerin antibody (82888-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells. This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 82888-3-RR (same clone as 82888-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-3-RR (Emerin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug EMD Recombinant antibody (82888-3-RR, Clone:230089B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82888-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82888-3-RR against Human EMD were performed. The affinity constant is 11.1 nM.

---

Informations sur le produit

82888-3-PBS cible Emerin dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Emerin Protéine recombinante Ag0236
• Nom complet: emerin
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC000738
• Symbole du gène: EMD
• Identification du gène (NCBI): 2010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}