Anticorps Recombinant de lapin anti-Emerin

• Phare
• Validé par KD/KO

Emerin Recombinant Antibody for WB, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra)

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230089B12

N° de cat : CL750-82888-3

Synonymes

EMD, STA, LEMD5, EDMD, 230089B12

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Galerie de données de validation

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  WB analysis using CL750-82888-3

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82888-3 (Emerin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293T using CL750-82888-3

  1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated Emerin Recombinant antibody (CL750-82888-3, Clone:230089B12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en FC (Intra): cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-82888-3 cible Emerin dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Emerin Protéine recombinante Ag0236
• Nom complet: emerin
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC000738
• Symbole du gène: EMD
• Identification du gène (NCBI): 2010
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

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