Anticorps Polyclonal de lapin anti-EML1

EML1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12765-1-PBS

Synonymes

HuEMAP-1, EMAP-1, EMAP, Echinoderm microtubule-associated protein-like 1

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Galerie de données de validation

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  WB analysis using 12765-1-AP (same clone as 12765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12765-1-AP (EML1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12765-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12765-1-AP (same clone as 12765-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12765-1-AP (EML1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12765-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 12765-1-AP (same clone as 12765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12765-1-AP (EML1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12765-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12765-1-AP (same clone as 12765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12765-1-AP (EML1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12765-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12765-1-PBS cible EML1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EML1 Protéine recombinante Ag3429
• Nom complet: echinoderm microtubule associated protein like 1
• Masse moléculaire calculée: 815 aa, 90 kDa
• Poids moléculaire observé: 90-92 kDa
• Numéro d’acquisition GenBank: BC033043
• Symbole du gène: EML1
• Identification du gène (NCBI): 2009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EML1 is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped.

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