Anticorps Polyclonal de lapin anti-EMR2/CD312

EMR2/CD312 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 32405-1-AP

Synonymes

EMR2, ADGRE2, Adhesion G protein-coupled receptor E2, CD312, EGF like module receptor 2

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Galerie de données de validation

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  WB analysis using 32405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32405-1-AP (EMR2/CD312 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 32405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32405-1-AP (EMR2/CD312 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32405-1-AP cible EMR2/CD312 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EMR2/CD312 Protéine recombinante Eg1997
• Nom complet: egf-like module containing, mucin-like, hormone receptor-like 2
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 75~90 kDa
• Numéro d’acquisition GenBank: NM_013447.4
• Symbole du gène: EMR2
• Identification du gène (NCBI): 30817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668). The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).

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