Anticorps Recombinant de lapin anti-EMR2

EMR2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242042E3

N° de cat : 84608-5-RR

Synonymes

EGF-like module receptor 2, EGF like module receptor 2, CD312, Adhesion G protein-coupled receptor E2, ADGRE2

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Galerie de données de validation

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  WB analysis using 84608-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84608-5-RR (EMR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84608-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84608-5-RR against Human EMR2 were performed. The affinity constant is 1.45 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84608-5-RR cible EMR2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: egf-like module containing, mucin-like, hormone receptor-like 2
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 75~90 kDa
• Numéro d’acquisition GenBank: NM_013447.3
• Symbole du gène: EMR2
• Identification du gène (NCBI): 30817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668).The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).

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