Anticorps Recombinant de lapin anti-ENDOG

ENDOG Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250804D2

N° de cat : 86122-1-PBS

Synonymes

EC:3.1.30.-, Endo G, Endonuclease G

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Galerie de données de validation

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  WB analysis using 86122-1-RR (same clone as 86122-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

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  WB analysis using 86122-1-RR (same clone as 86122-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02519-1

  The mean ENDOG concentration was determined to be 1,103.00 ng/mL in Ramos cell extract based on a 1.40 mg/mL extract load, 1,271.00 ng/mL in HepG2 cell extract based on a 1.3 mg/mL extract load and 967.00 ng/mL in HL-60 cell extract based on a 3.7 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02519-1

  Sandwich ELISA standard curve of MP02519-1, Human ENDOG Recombinant Matched Antibody Pair - PBS only. 86122-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17739. 86122-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.

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  IHC staining of mouse kidney using 86122-1-RR (same clone as 86122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86122-1-RR (ENDOG antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86122-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86122-1-RR against Human ENDOG were performed. The affinity constant is 0.659 nM.

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Informations sur le produit

86122-1-PBS cible ENDOG dans les applications de WB, IHC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ENDOG Protéine recombinante Ag17739
• Nom complet: endonuclease G
• Masse moléculaire calculée: 297 aa, 33 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC016351
• Symbole du gène: ENDOG
• Identification du gène (NCBI): 2021
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.

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