Anticorps Polyclonal de lapin anti-ENGASE

ENGASE Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31784-1-PBS

Synonymes

EC:3.2.1.96, Cytosolic endo-beta-N-acetylglucosaminidase

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Galerie de données de validation

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  IHC staining of mouse spleen using 31784-1-AP (same clone as 31784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31784-1-AP (same clone as 31784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 31784-1-AP (same clone as 31784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 31784-1-AP (same clone as 31784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 31784-1-AP (same clone as 31784-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ENGASE antibody (31784-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31784-1-PBS cible ENGASE dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ENGASE Protéine recombinante Ag34633
• Nom complet: endo-beta-N-acetylglucosaminidase
• Masse moléculaire calculée: 84 kDa
• Numéro d’acquisition GenBank: NM_001042573
• Symbole du gène: ENGASE
• Identification du gène (NCBI): 64772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Endo-β-N-acetylglucosaminidase (ENGASE) is an enzyme that belongs to glycoside hydrolase (GH) families 18 and 85, and is widely distributed in bacteria, fungi, and higher-order species. ENGASE hydrolyzes the β1-4 linkage of the N, N-diacetylchitobiose (GlcNAcβ1-4GlcNAc) region of the N-linked glycans present on glycoproteins (PMID: 36435009). ENGASE is widely expressed, with higher expression in thymus and spleen (Refer to Uniprot).

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