Anticorps Monoclonal anti-ENO3

ENO3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C12G3

N° de cat : 68147-1-PBS

Synonymes

Beta enolase, ENO3, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

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Galerie de données de validation

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  WB analysis using 68147-1-Ig (same clone as 68147-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68147-1-Ig (ENO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68147-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68147-1-Ig (same clone as 68147-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ENO3 antibody (68147-1-Ig, Clone: 2C12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68147-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68147-1-PBS cible ENO3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ENO3 Protéine recombinante Ag9642
• Nom complet: enolase 3 (beta, muscle)
• Masse moléculaire calculée: 434 aa, 47 kDa
• Poids moléculaire observé: 42-47 kDa
• Numéro d’acquisition GenBank: BC017249
• Symbole du gène: ENO3
• Identification du gène (NCBI): 2027
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Enolase 3 (ENO3) encodes the β-subunit of enolase, which is distributed in various tissues, including liver, lung, skeletal, and heart. ENO3 plays an important role in both glycogen and cholesterol metabolism. It has been demonstrated that ENO3 deficiency may lead to metabolic myopathies, and ENO3 speeds up hepatic cholesterol ester cumulation induced via the mediation of cholesteryl ester generation.Apart from these, ENO3 has also been involved in various tumors, though the results are contradictory. For example, ENO3 is up-regulated in STK11 mutant pulmonary carcinoma and colorectal carcinoma, while down-regulated in pancreatic cancer and hepatocellular carcinomas. ENO3 has 3 isoforms with the molecular mass of 42-47 kDa. (PMID: 33987359, 35004693)

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