Anticorps Monoclonal anti-ENOPH1

ENOPH1 Monoclonal Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F1E11

N° de cat : 68179-1-PBS

Synonymes

MASA, 1F1E11, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, E1, EC:3.1.3.77

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Galerie de données de validation

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  WB analysis using 68179-1-Ig (same clone as 68179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68179-1-Ig (ENOPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68179-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68179-1-Ig (same clone as 68179-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ENOPH1 Monoclonal antibody (68179-1-Ig, Clone:1F1E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68179-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50127-1

  Cytometric bead array standard curve of MP50127-1, ENOPH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68179-1-PBS. Detection antibody: 68179-2-PBS. Standard:Ag12553. Range: 0.781-100 ng/mL.

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Informations sur le produit

68179-1-PBS cible ENOPH1 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ENOPH1 Protéine recombinante Ag12553
• Nom complet: enolase-phosphatase 1
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 29-35 kDa
• Numéro d’acquisition GenBank: BC065815
• Symbole du gène: ENOPH1
• Identification du gène (NCBI): 58478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.

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