Anticorps Recombinant de lapin anti-ENOSF1

ENOSF1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240796D2

N° de cat : 83769-2-PBS

Synonymes

TYMSAS, rTS, L-fuconate dehydratase, enolase superfamily member 1, EC:4.2.1.68

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Galerie de données de validation

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  WB analysis using 83769-2-RR (same clone as 83769-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83769-2-RR (ENOSF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83769-2-PBS in a different storage buffer formulation.

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  FC experiment of Hela using 83769-2-RR (same clone as 83769-2-PBS)

  1x10^6 Hela cells were intracellularly stained with 0.25 ug ENOSF1 Recombinant antibody (83769-2-RR, Clone:240796D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83769-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00758-1

  Cytometric bead array standard curve of MP00758-1, ENOSF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83769-1-PBS. Detection antibody: 83769-2-PBS. Standard: Ag34665. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83769-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83769-2-RR against Human ENOSF1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83769-2-PBS cible ENOSF1 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ENOSF1 Protéine recombinante Ag34665
• Nom complet: enolase superfamily member 1
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC001285
• Symbole du gène: ENOSF1
• Identification du gène (NCBI): 55556
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).

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