Anticorps Recombinant de lapin anti-ENPEP

ENPEP Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250264B6

N° de cat : 85963-1-RR

Synonymes

AP A, APA, AP-A, CD249, EAP

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Galerie de données de validation

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  WB analysis using 85963-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 85963-1-RR

  rat lung tissue were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 85963-1-RR

  human kidney tissue were subjected to SDS PAGE followed by western blot with 85963-1-RR (ENPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85963-1-RR cible ENPEP dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ENPEP Protéine recombinante Eg2736
• Nom complet: glutamyl aminopeptidase (aminopeptidase A)
• Masse moléculaire calculée: 109 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_001977.3
• Symbole du gène: ENPEP
• Identification du gène (NCBI): 2028
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.

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