Anticorps Recombinant de lapin anti-ENPEP

ENPEP Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250659C1

N° de cat : 98489-3-RR

Synonymes

Aminopeptidase A, AP-A, BP-1/6C3 antigen, CD249, EAP

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using 98489-3-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse ENPEP Rabbit RecAb (98489-3-RR, Clone:250659C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD45R. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98489-3-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse ENPEP Rabbit RecAb (98489-3-RR, Clone: 250659C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD45R. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98489-3-RR cible ENPEP dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ENPEP Protéine recombinante Eg4233
• Nom complet: glutamyl aminopeptidase
• Masse moléculaire calculée: 108 kDa
• Numéro d’acquisition GenBank: NM_007934.3
• Symbole du gène: Enpep
• Identification du gène (NCBI): 13809
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.

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