Anticorps Polyclonal de lapin anti-ENTPD8

ENTPD8 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 21010-1-AP

Synonymes

E NTPDase 8, ENTPD8, GLSR2492, NTPDase 8, NTPDase8, UNQ2492

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Galerie de données de validation

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  WB analysis using 21010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 21010-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21010-1-AP (ENTPD8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2 incubées à 37 °C, tissu hépatique de rat, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21010-1-AP cible ENTPD8 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ENTPD8 Protéine recombinante Ag15224
• Nom complet: ectonucleoside triphosphate diphosphohydrolase 8
• Masse moléculaire calculée: 495 aa, 54 kDa
• Poids moléculaire observé: 70 kDa, 54 kDa
• Numéro d’acquisition GenBank: BC141810
• Symbole du gène: ENTPD8
• Identification du gène (NCBI): 377841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENTPD8 (Ectonucleoside triphosphate diphosphohydrolase 8) is also named as E-NTPDase 8, NTPDase8 and belongs to the GDA1/CD39 NTPase family. NTPDase8 is the canalicular ecto-ATPase/ATPDase and is responsible for the main hepatic NTP-Dase activity(PMID:17095758). The estimated molecular mass of expressed human ENTPD8 is 70-85 kDa which is N-glycosylated and after deglycosylation, it has a nearly identical molecular mass, 54 kDa(PMID:16752921;7781224). It has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 49 kDa. This antibody is specific to ENTPD8.

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