Anticorps Recombinant de lapin anti-EOMES/TBR2

EOMES/TBR2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241036D9

N° de cat : 83945-5-RR

Synonymes

EOMES, TBR2, 241036D9, Eomesodermin homolog, T box brain protein 2

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Galerie de données de validation

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  WB analysis using 83945-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83945-5-RR (EOMES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83945-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83945-5-RR (EOMES/TBR2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83945-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83945-5-RR (EOMES/TBR2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 83945-5-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using EOMES antibody (83945-5-RR, Clone: 241036D9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83945-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83945-5-RR against Human EOMES were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: NK-92 cells,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83945-5-RR cible EOMES/TBR2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EOMES/TBR2 Protéine recombinante Ag27634
• Nom complet: eomesodermin homolog (Xenopus laevis)
• Masse moléculaire calculée: 73 aa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: NM_001278182
• Symbole du gène: EOMES
• Identification du gène (NCBI): 8320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EOMES/TRB2 (also named Eomesodermin/Tbr2) encode T-box transcription factor expressed highly in the intermediate progenitor stage of the developing neuron. During migration of the neurons, radial glia divide and migrate towards the surface of the cerebral cortex, there are three stages of cellular development: radial glia, intermediate progenitors, and postmitotic projection neurons. Radial glia express Pax6, while intermediate progenitor cells express Eomesodermin/Tbr2, and postmitotic projection neurons express Tbr1 (PMID:15634788; PMID:16320258). This process also called neurogenesis, and demonstrated that Eomesodermin/Tbr2 has been implicated in neurodevelopment. According to the mouse model, several articles showed that EOMES/TRB2 KO mice having proliferating cells decreased and having smaller upper cortical layers and a smaller sub ventricular zone in the brain (PMID:18940588). What's more, Eomesodermin/Tbr2 has also been found participating in immune response (PMID:20713880).

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