Anticorps Polyclonal de lapin anti-EP400

EP400 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13858-1-PBS

Synonymes

CAGH32, E1A-binding protein p400, EC:3.6.4.-, EP 400, hDomino

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Galerie de données de validation

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  IP experiment of HEK-293 using 13858-1-AP (same clone as 13858-1-PBS)

  IP result of anti-EP400 (IP:13858-1-AP, 4ug; Detection:13858-1-AP 1:500) with HEK-293 cells lysate 1440 ug. This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 13858-1-AP (same clone as 13858-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13858-1-AP (EP400 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13858-1-AP (same clone as 13858-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EP400 antibody (13858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 13858-1-AP (same clone as 13858-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EP400 antibody (13858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13858-1-PBS cible EP400 dans les applications de IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EP400 Protéine recombinante Ag4832
• Nom complet: E1A binding protein p400
• Masse moléculaire calculée: 3160 aa, 344 kDa
• Poids moléculaire observé: 344 kDa
• Numéro d’acquisition GenBank: BC037208
• Symbole du gène: EP400
• Identification du gène (NCBI): 57634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

E1A binding protein p400 (EP400) is a SWR1-class ATP-dependent chromatin remodeling protein originally identified as an interacting partner for the adenovirus E1A protein required for viral replication and cellular transformation. EP400 plays a crucial role in promoting double-strand break (DSB) repair through homologous recombination (PMID: 26669263, 37922899). Depletion of Ep400 in oocytes led to accumulated DSBs, causing abnormal aggregation and dysfunction of cytoplasmic organelles, resulting in impaired oocyte development and declined oocyte quality (PMID: 38493496).

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