Anticorps Polyclonal de lapin anti-EPAC1

EPAC1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris, Hamster

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12572-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of CHO using 12572-1-AP (same clone as 12572-1-PBS)

  CHO cells were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  WB analysis using 12572-1-AP (same clone as 12572-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 12572-1-AP (same clone as 12572-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 12572-1-AP (same clone as 12572-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12572-1-PBS cible EPAC1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris, Hamster

• Réactivité: Humain, rat, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPAC1 Protéine recombinante Ag3240
• Nom complet: Rap guanine nucleotide exchange factor (GEF) 3
• Masse moléculaire calculée: 881 aa, 99 kDa
• Poids moléculaire observé: 99-104 kDa
• Numéro d’acquisition GenBank: BC017728
• Symbole du gène: EPAC1/RAPGEF3
• Identification du gène (NCBI): 10411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPAC1 functions as a guanine nucleotide exchange factor (GEF), activating small GTPases like Rap1 and Rap2, leading to diverse cellular response. Epac proteins are cAMP sensors that regulate several pivotal cellular processes, including calcium handling, cell proliferation, cell survival, cell differentiation, cell polarization, cell adhesion events, gene transcription, secretion, ion transport, and neuronal signaling. It is a potential target for therapeutic intervention, such as metabolic disorders, cardiovascular diseases (PMID: 20007405, PMID: 20421979). EPAC1 also enhances brown fat growth and beige adipogenesis (PMID: 38195707).

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