Anticorps Recombinant de lapin anti-HIF2α/EPAS1

HIF2α/EPAS1 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240736F2

N° de cat : 83790-1-PBS

Synonymes

EPAS1, 240736F2, Basic-helix-loop-helix-PAS protein MOP2, bHLHe73, Class E basic helix-loop-helix protein 73

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Galerie de données de validation

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  WB analysis of A549 using 83790-1-RR (same clone as 83790-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 83790-1-RR (EPAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83790-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 83790-1-RR (same clone as 83790-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 83790-1-RR (EPAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83790-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83790-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83790-1-RR against Human HIF2α/EPAS1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83790-1-PBS cible HIF2α/EPAS1 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HIF2α/EPAS1 Protéine recombinante Ag15199
• Nom complet: endothelial PAS domain protein 1
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC051338
• Symbole du gène: HIF2α
• Identification du gène (NCBI): 2034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HIF2A, also named as EPAS1, is a 870 amino acid protein, which is expressed in most tissues, with highest levels in placenta, lung and heart. HIF2A colocalizes with HIF3A in the nucleus and speckles. HIF2A as a transcription factor involves in the induction of oxygen regulated genes. HIF2A binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. HIF2A regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. HIF2A may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. The calculated molecular weight of HIF2A is 96 kDa, but In normoxia, HIF2A is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. The modified HIf2A is about 100-120 kDa.

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