Anticorps Recombinant de lapin anti-EPB41L3

• Phare
• Validé par KD/KO

EPB41L3 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230501D7

N° de cat : 83275-2-PBS

Synonymes

DAL-1, DAL1, Band 4.1-like protein 3, N-terminally processed, Band 4.1-like protein 3, 4.1B

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Galerie de données de validation

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  WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 83275-2-RR (EPB41L3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

  WB result of EPB41L3 antibody (83275-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPB41L3 transfected U2OS cells. This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83275-2-RR (same clone as 83275-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug EPB41L3 Recombinant antibody (83275-2-RR, Clone:230501D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83275-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83275-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83275-2-RR against Human EPB41L3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83275-2-PBS cible EPB41L3 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPB41L3 Protéine recombinante Ag1089
• Nom complet: erythrocyte membrane protein band 4.1-like 3
• Masse moléculaire calculée: 121 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC008377
• Symbole du gène: EPB41L3
• Identification du gène (NCBI): 23136
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPB41L3, also known as 4.1B and DAL1, belongs to the protein 4.1 family. It is a tumor suppressor that inhibits cell proliferation and promotes apoptosis. EPB41L3 modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5 (PMID: 15334060, 15737618). DAL1 loss is an early event in meningioma tumorigenesis (PMID: 10888600).

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