Anticorps Recombinant de lapin anti-EPB41L4B

EPB41L4B Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242186G4

N° de cat : 84732-2-RR

Synonymes

FERM containing protein CG1, EHM2, CG1, Band 4.1 like protein 4B, 242186G4

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Galerie de données de validation

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  WB analysis using 84732-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84732-2-RR (EPB41L4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84732-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84732-2-RR against Human EPB41L4B were performed. The affinity constant is 6.86 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HaCaT, cellules HEK-293T, cellules HepG2, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84732-2-RR cible EPB41L4B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPB41L4B Protéine recombinante Ag36353
• Nom complet: erythrocyte membrane protein band 4.1 like 4B
• Masse moléculaire calculée: 99kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: NM_00138562
• Symbole du gène: EPB41L4B
• Identification du gène (NCBI): 54566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 4.1 proteins, encoded by the EPB41 (erythrocyte protein band 4.1) genes, are components of the cortical cytoskeleton underlying the cell membrane. The family of 4.1 proteins consists of the eponymous 4.1R protein first identified in erythrocytes (gene: EPB41), 4.1N (EPB41L1), 4.1G (EPB41L2), 4.1B (EPB41L3) as well as the less closely related members NBL4 (EPB41L4A), EHM2 (EPB41L4B) and EPB41L5 (EPB41L5). EHM2 is conspicuous in tumor cells with high migratory potential, such as metastatic melanoma and fibrosarcoma cells. In prostate cancer, EHM2 has been reported to be overexpressed and to diminish adhesion of prostate cancer cells to collagen. (PMID: 20860828)

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