Anticorps Recombinant de lapin anti-EPB42

EPB42 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242412F4

N° de cat : 84921-3-PBS

Synonymes

242412F4, E42P, Erythrocyte protein 4.2, P4.2, PA

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Galerie de données de validation

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  WB analysis of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84921-3-RR (EPB42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84921-3-RR (same clone as 84921-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84921-3-RR (EPB42 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

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  IF Staining of HEL using 84921-3-RR (same clone as 84921-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using EPB42 antibody (84921-3-RR, Clone: 242412F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84921-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01708-1

  Cytometric bead array standard curve of MP01708-1, EPB42 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84921-2-PBS. Detection antibody: 84921-3-PBS. Standard: Ag23556. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01708-2

  Cytometric bead array standard curve of MP01708-2, EPB42 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84921-1-PBS. Detection antibody: 84921-3-PBS. Standard: Ag23556. Range: 1.25-80 ng/mL.

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Informations sur le produit

84921-3-PBS cible EPB42 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPB42 Protéine recombinante Ag23556
• Nom complet: erythrocyte membrane protein band 4.2
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC096093
• Symbole du gène: EPB42
• Identification du gène (NCBI): 2038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.

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