Anticorps Polyclonal de lapin anti-EPCAM/CD326

• Phare
• Validé par KD/KO

EPCAM/CD326 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(90)

Avis (1)

N° de cat : 21050-1-AP

Synonymes

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

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Galerie de données de validation

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  WB analysis of A431 using 21050-1-AP

  WB result of EPCAM antibody (21050-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

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  WB analysis using 21050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse lung using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using EPCAM antibody (21050-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21050-1-AP (EPCAM antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HT-29 using 21050-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HT-29 using 21050-1-AP

  1x10^6 HT-29 cells were surface stained with 0.5 ug EPCAM/CD326 Polyclonal antibody (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.5 ug Control Antibody (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules A431, tissu de côlon de rat, tissu de côlon de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de côlon de souris, tissu de côlon humain, tissu d'estomac de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris, cellules MCF-7, tissu pulmonaire de souris
• Résultats positifs en IF-Fro: tissu de côlon de souris,
• Résultats positifs en IF/ICC: cellules HT-29, cellules MCF-7
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 45 publications below
• IHC: See 17 publications below
• IF: See 34 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

21050-1-AP cible EPCAM/CD326 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, FC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPCAM/CD326 Protéine recombinante Ag15393
• Nom complet: epithelial cell adhesion molecule
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC014785
• Symbole du gène: EPCAM
• Identification du gène (NCBI): 4072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Publications

Species	Application	Title
human	IF	Cancer Cell; Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis; Authors - Jiang Chang
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
human,mouse	IF	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
mouse	IHC	Cancer Cell; Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression.; Authors - Chunxiao Xu
mouse	IF	Gut; Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.; Authors - Yunben Yang
	IF	Autophagy; Neutrophil-derived serine proteases induce FOXA2-mediated autophagy dysfunction and exacerbate colitis-associated carcinogenesis via F2RL1/protease-activated receptor 2; Authors - Junhu Yuan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrew (Verified Customer) (07-12-2024)

Got a clean band at the desired molecular weight for EPCAM on the first attempt of using this primary antibody for Western Blot. Also, there was very little background. Overall, very happy with this product.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HGSOC Commercial Cell Line

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