Anticorps Monoclonal anti-EPCAM/CD326

• Phare
• Validé par KD/KO

EPCAM/CD326 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2A2D5

N° de cat : 66316-1-PBS

Synonymes

EPCAM, 2A2D5, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1

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Galerie de données de validation

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  WB analysis of A431 using 66316-1-Ig (same clone as 66316-1-PBS)

  WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  WB analysis using 66316-1-Ig (same clone as 66316-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66316-1-Ig (same clone as 66316-1-PBS)

  T-47D, MCF-7 and A431 cells were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IHC staining of Human colon using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human colon tissue slide using 66316-1-Ig (EPCAM/CD326 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human stomach cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66316-1-PBS cible EPCAM/CD326 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EPCAM/CD326 Protéine recombinante Ag15393
• Nom complet: epithelial cell adhesion molecule
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC014785
• Symbole du gène: EPCAM
• Identification du gène (NCBI): 4072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

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