Anticorps Recombinant de lapin anti-EPCAM/CD326

EPCAM/CD326 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241243B10

N° de cat : 84073-6-PBS

Synonymes

EPCAM, 241243B10, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1

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Galerie de données de validation

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  WB analysis using 84073-6-RR (same clone as 84073-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84073-6-RR (same clone as 84073-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84073-6-RR (same clone as 84073-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84073-6-RR (same clone as 84073-6-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM/CD326 antibody (84073-6-RR, Clone: 241243B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84073-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84073-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84073-6-RR against Human EPCAM/CD326 were performed. The affinity constant is 2.56 nM.

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Informations sur le produit

84073-6-PBS cible EPCAM/CD326 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPCAM/CD326 Protéine recombinante Eg1370
• Nom complet: epithelial cell adhesion molecule
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: NM_002354.3
• Symbole du gène: EPCAM
• Identification du gène (NCBI): 4072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

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