Anticorps Recombinant de lapin anti-EPCAM/CD326

EPCAM/CD326 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

241243B10

N° de cat : 84073-6-RR

Synonymes

EPCAM, 241243B10, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1

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Galerie de données de validation

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  WB analysis using 84073-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84073-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84073-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84073-6-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 84073-6-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM/CD326 antibody (84073-6-RR, Clone: 241243B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84073-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84073-6-RR against Human EPCAM/CD326 were performed. The affinity constant is 2.56 nM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HaCaT, cellules HCT 116, cellules MCF-7
• Résultats positifs en IHC: human ovary cancer tissue, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

84073-6-RR cible EPCAM/CD326 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPCAM/CD326 Protéine recombinante Eg1370
• Nom complet: epithelial cell adhesion molecule
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: NM_002354.3
• Symbole du gène: EPCAM
• Identification du gène (NCBI): 4072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Publications

Species	Application	Title
human	IF	J Transl Med; Tumor microenvironment remodeling after neoadjuvant chemoradiotherapy in local advanced rectal cancer revealed by single-cell RNA sequencing; Authors - Wenzhao Su

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