Anticorps Polyclonal de lapin anti-EPG5

EPG5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27666-1-PBS

Synonymes

HEEW1, KIAA1632, UPF0493 protein KIAA1632

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Galerie de données de validation

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  WB analysis using 27666-1-AP (same clone as 27666-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27666-1-AP (EPG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27666-1-AP (same clone as 27666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27666-1-AP (same clone as 27666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 27666-1-AP (same clone as 27666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27666-1-AP (EPG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27666-1-PBS cible EPG5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPG5 Protéine recombinante Ag24801
• Nom complet: KIAA1632
• Masse moléculaire calculée: 2579 aa, 292 kDa
• Poids moléculaire observé: 270-290 kDa
• Numéro d’acquisition GenBank: BC130614
• Symbole du gène: EPG5
• Identification du gène (NCBI): 57724
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPG5, a metazoan-specific autophagy protein, regulates autophagy by promoting fusion of autophagosomes with lysosomes and/or late endosomes. EPG5 regulates the stability and the assembly of STX17-SNAP29-VAMP8 trans-SNARE complexes. Loss of function of EPG5 causes non-specific fusion of autophagosomes with other endocytic vesicles, resulting in formation of non-degradative enlarged vesicles. EPG5 expression in human pluripotent stem cells like ESC and iPSC is higher than that of human somatic cells.(PMID: 30931944, PMID: 27588602)

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