Anticorps Polyclonal de lapin anti-EPHA1-specific

EPHA1-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 18698-1-AP

Synonymes

EPHA1, EPHA1-special, EPH tyrosine kinase 1, EPH tyrosine kinase, EPH receptor A1

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Galerie de données de validation

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  WB analysis of A549 using 18698-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18698-1-AP (EPHA1-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18698-1-AP (EPHA1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 18698-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 18698-1-AP (EPHA1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

18698-1-AP cible EPHA1-specific dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: EPH receptor A1
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC130291
• Symbole du gène: EPHA1
• Identification du gène (NCBI): 2041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.

Publications

Species	Application	Title
human,mouse	WB,IP,IHC	EMBO J; Ephrin A1 functions as a ligand of EGFR to promote EMT and metastasis in gastric cancer; Authors - Shuang Li ; KD Validated

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