Anticorps Monoclonal anti-EPHA1-specific

EPHA1-specific Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B7C3

N° de cat : 60155-2-PBS

Synonymes

EPH, EPH receptor A1, EPHA1, EPHA1-specific, Ephrin type A receptor 1, EPHT, EPHT1

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Galerie de données de validation

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  WB analysis of HeLa using 60155-2-Ig (same clone as 60155-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60155-2-Ig (same clone as 60155-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60155-2-Ig (same clone as 60155-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

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Informations sur le produit

60155-2-PBS cible EPHA1-specific dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: EPH receptor A1
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 108 kDa
• Numéro d’acquisition GenBank: BC130291
• Symbole du gène: EPHA1
• Identification du gène (NCBI): 2041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.

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