Anticorps Monoclonal anti-EPHA2

• Phare
• Validé par KD/KO

EPHA2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A9C3

N° de cat : 66736-1-PBS

Synonymes

1A9C3, EC:2.7.10.1, ECK, EPH receptor A2, Ephrin type A receptor 2

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Galerie de données de validation

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  WB analysis using 66736-1-Ig (same clone as 66736-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66736-1-Ig (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66736-1-Ig (same clone as 66736-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPHA2 antibody (66736-1-Ig, Clone: 1A9C3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66736-1-PBS cible EPHA2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EPHA2 Protéine recombinante Ag22566
• Nom complet: EPH receptor A2
• Masse moléculaire calculée: 976 aa, 108 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC037166
• Symbole du gène: EPHA2
• Identification du gène (NCBI): 1969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).

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