Anticorps Monoclonal anti-EPHA7

• Phare
• Validé par KD/KO

EPHA7 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1A7E5

N° de cat : 66667-1-Ig

Synonymes

EPH homology kinase 3, EK11, EHK-3, EHK3, EHK 3

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Galerie de données de validation

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  WB analysis of human brain using 66667-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66667-1-Ig

  Untreated and PNGase F-treated lysates of pig brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of rat brain using 66667-1-Ig

  Untreated and PNGase F-treated lysates of rat brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of pig brain using 66667-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66667-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 66667-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66667-1-Ig (EPHA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de porc, tissu cérébral de rat, tissu de cervelet de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

66667-1-Ig cible EPHA7 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EPHA7 Protéine recombinante Ag3813
• Nom complet: EPH receptor A7
• Masse moléculaire calculée: 998 aa, 112 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC027940
• Symbole du gène: EPHA7
• Identification du gène (NCBI): 2045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. EPHA7 plays a role in corticogenesis processes, determines brain size and shape, and is involved in development of the central nervous system (PMID:34176129). In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor. It can participate in cell proliferation, migration and apoptosis through various mechanisms, and afect tumor diferentiation, staging and prognosis. EphA7 may be a potential diagnostic marker and tumor treatment target (PMID:35112312).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Transcriptomics-Based Liquid Biopsy for Early Detection of Recurrence in Locally Advanced Gastric Cancer; Authors - Ping'an Ding ; KD Validated
mouse	WB	Cell Rep; Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells; Authors - Fenghua Qian
mouse	WB	Int J Mol Sci; Fucoxanthin Prevents Pancreatic Tumorigenesis in C57BL/6J Mice That Received Allogenic and Orthotopic Transplants of Cancer Cells.; Authors - Wataru Murase

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