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Anticorps Polyclonal de lapin anti-EPHB3

EPHB3 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21382-1-PBS

Synonymes

EK2, EPH like kinase 2, EPH receptor B3, Ephrin type B receptor 3, ETK2

Galerie de données de validation

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using EPHB3 Polyclonal antibody (21382-1-AP)

    IHC staining of mouse brain using 21382-1-AP (same clone as 21382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21382-1-AP (EPHB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EPHB3 Polyclonal antibody (21382-1-AP)

    IHC staining of mouse brain using 21382-1-AP (same clone as 21382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21382-1-AP (EPHB3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using EPHB3 Polyclonal antibody (21382-1-AP)

    IF Staining of Jurkat using 21382-1-AP (same clone as 21382-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using EPHB3 antibody (21382-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

Informations sur le produit

21382-1-PBS cible EPHB3 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène EPHB3 Protéine recombinante Ag16016
Nom complet EPH receptor B3
Masse moléculaire calculée 998 aa, 110 kDa
Numéro d’acquisition GenBankBC052968
Symbole du gène EPHB3
Identification du gène (NCBI) 2049
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPHB3 is a member of the Eph receptor tyrosine kinase family. The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). The EPHB3 protein has multiple functions in the nervous system, including regulating neural networks, participating in epileptic activity, and playing a role in repair after spinal cord injury.

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