Anticorps Polyclonal de lapin anti-EPHB4

• Phare
• Validé par KD/KO

EPHB4 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 20883-1-AP

Synonymes

EPH receptor B4, EPHB4, Ephrin type B receptor 4, HTK, MYK1, TYRO11, Tyrosine protein kinase TYRO11

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Galerie de données de validation

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  WB analysis using 20883-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 20883-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 20883-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 20883-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 20883-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20883-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 20883-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 20883-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HT-29 using 20883-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPHB4 antibody (20883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 20883-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug EPHB4 antibody (20883-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules DU 145, cellules HeLa, cellules HT-1080, cellules PC-3, tissu testiculaire de rat
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu pancréatique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HT-29,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 11 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Informations sur le produit

20883-1-AP cible EPHB4 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPHB4 Protéine recombinante Ag10033
• Nom complet: EPH receptor B4
• Masse moléculaire calculée: 987 aa, 108 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC052804
• Symbole du gène: EPHB4
• Identification du gène (NCBI): 2050
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Small; Specific Photothermal Ablation Therapy of Endometriosis by Targeting Delivery of Gold Nanospheres.; Authors - Xiaomeng Guo
human	IF	Drug Deliv; Near infrared light mediated photochemotherapy for efficiently treating deep orthotopic tumors guided by ultrasound imaging.; Authors - Zuhua Wang
	WB,IHC	J Cell Physiol; Intermittent parathyroid hormone promotes cementogenesis via ephrinB2-EPHB4 forward signaling.; Authors - Tiancheng Li
human	WB	J Cell Mol Med; Kinome multigenic panel identified novel druggable EPHB4-V871I somatic variant in high-risk neuroblastoma.; Authors - Immacolata Andolfo
human	FC	Phytomedicine; Sanguinarine combats hypoxia-induced activation of EphB4 and HIF-1α pathways in breast cancer.; Authors - Qi Su
rat	IHC	Commun Biol; The application of tissue-engineered fish swim bladder vascular graft.; Authors - Hualong Bai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marsilius (Verified Customer) (04-13-2021)

This antibody is useful for staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: brain

EPHB4 Antibody Immunofluorescence validation (1:100 dilution) in brain (Cat no:20883-1-AP)

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