Anticorps Monoclonal anti-EPHX1

• Phare
• Validé par KD/KO

EPHX1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B6A2

N° de cat : 66982-1-PBS

Synonymes

EPHX, EPHX1, EPOX, Epoxide hydratase, Epoxide hydrolase 1, MEH, Microsomal epoxide hydrolase

---

Galerie de données de validation

• 

  WB analysis using 66982-1-Ig (same clone as 66982-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66982-1-Ig (same clone as 66982-1-PBS)

  WB result of EPHX1 antibody (66982-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX1 transfected HeLa cells. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66982-1-Ig (same clone as 66982-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 66982-1-Ig (same clone as 66982-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66982-1-PBS cible EPHX1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EPHX1 Protéine recombinante Ag25820
• Nom complet: epoxide hydrolase 1, microsomal (xenobiotic)
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC003567
• Symbole du gène: EPHX1
• Identification du gène (NCBI): 2052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPHX1, also known as EPHX and EPOX, belongs to the peptidase S33 family. EPHX1 is an evolutionarily highly conserved biotransformation enzyme expressed in nearly all tissues and localized mainly in the microsomal fraction of the endoplasmic reticulum of eukaryotic cells. EPHX1 plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. The calculated molecular weight of EPHX1 is 53 kDa (PMID: 26216302).

{{ptg:RelatedPrimaryAntibodies}}