Anticorps Monoclonal anti-EPHX2

• Phare
• Validé par KD/KO

EPHX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4H6C8

N° de cat : 67322-1-PBS

Synonymes

SEH, Lipid-phosphate phosphatase, Cytosolic epoxide hydrolase 2, CEH, Bifunctional epoxide hydrolase 2

---

Galerie de données de validation

• 

  WB analysis of A549 using 67322-1-Ig (same clone as 67322-1-PBS)

  WB result of EPHX2 antibody (67322-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX2 transfected A549 cells. This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67322-1-Ig (same clone as 67322-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67322-1-Ig (EPHX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67322-1-Ig (same clone as 67322-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 10x lens). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67322-1-Ig (same clone as 67322-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67322-1-Ig (EPHX2 antibody) at dilution of 1:4000 (under 40x lens). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 67322-1-Ig (same clone as 67322-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using EPHX2 antibody (67322-1-Ig, Clone: 4H6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67322-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67322-1-PBS cible EPHX2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EPHX2 Protéine recombinante Ag4870
• Nom complet: epoxide hydrolase 2, cytoplasmic
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 56-63 kDa
• Numéro d’acquisition GenBank: BC013874
• Symbole du gène: EPHX2
• Identification du gène (NCBI): 2053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

{{ptg:RelatedPrimaryAntibodies}}