Anticorps Monoclonal anti-EPLIN

• Phare
• Validé par KD/KO

EPLIN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6E6E6

N° de cat : 66071-1-PBS

Synonymes

LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624

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Galerie de données de validation

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  WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

  WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66071-1-Ig (same clone as 66071-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66071-1-Ig (same clone as 66071-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66071-1-PBS cible EPLIN dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EPLIN Protéine recombinante Ag13288
• Nom complet: LIM domain and actin binding 1
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90 kDa, 110 kDa
• Numéro d’acquisition GenBank: BC001247
• Symbole du gène: EPLIN
• Identification du gène (NCBI): 51474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

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