Anticorps Monoclonal anti-EPO/Erythropoietin

EPO/Erythropoietin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11F3

N° de cat : 66975-1-PBS

Synonymes

EPO, MVCD2, erythropoietin, Epoetin, EP

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Galerie de données de validation

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  WB analysis of Recombinant protein using 66975-1-Ig (same clone as 66975-1-PBS)

  50 ng recombinant human EPO (cat.NO. HZ-1168) was subjected to SDS-PAGE under reducing condition followed by western blot with 66975-1-Ig (EPO antibody) at dilution of 1:10000. This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66975-1-Ig (same clone as 66975-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EPO antibody (66975-1-Ig, Clone: 1A11F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66975-1-PBS cible EPO/Erythropoietin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EPO/Erythropoietin Protéine recombinante Ag23054
• Nom complet: erythropoietin
• Masse moléculaire calculée: 193 aa, 21 kDa
• Numéro d’acquisition GenBank: BC093628
• Symbole du gène: EPO/Erythropoietin
• Identification du gène (NCBI): 2056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

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