Anticorps Recombinant de lapin anti-EPPK1

EPPK1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241633B5

N° de cat : 84344-2-PBS

Synonymes

EPPK, 241633B5, EPIPL, EPIPL1, Epiplakin

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Galerie de données de validation

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  WB analysis of L02 using 84344-2-RR (same clone as 84344-2-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 84344-2-RR (EPPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84344-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84344-2-RR (same clone as 84344-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EPPK1 antibody (84344-2-RR, Clone: 241633B5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84344-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 84344-2-RR (same clone as 84344-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84344-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84344-2-RR (same clone as 84344-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84344-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01220-1

  Cytometric bead array standard curve of MP01220-1, EPPK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84344-3-PBS. Detection antibody: 84344-2-PBS. Standard: Ag35749. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01220-2

  Cytometric bead array standard curve of MP01220-2, EPPK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84344-4-PBS. Detection antibody: 84344-2-PBS. Standard: Ag35749. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84344-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84344-2-RR against Human EPPK1 were performed. The affinity constant is 1.61 nM.

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Informations sur le produit

84344-2-PBS cible EPPK1 dans les applications de WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPPK1 Protéine recombinante Ag35749
• Nom complet: epiplakin 1
• Masse moléculaire calculée: 555 kDa
• Poids moléculaire observé: 500-600 kDa
• Numéro d’acquisition GenBank: NM_031308.3
• Symbole du gène: EPPK1
• Identification du gène (NCBI): 83481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Epplakin 1 (EPPK1), belonging to the plakin family, is involved in connecting intermediate filaments and regulating their reorganization in stress response. Studies have shown that EPPK1 is involved in the progression of various cancers, such as liver cancer, cervical cancer, and bladder urothelial carcinoma.

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