Anticorps Recombinant de lapin anti-EPPK1

EPPK1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241633B5

N° de cat : 84344-2-RR

Synonymes

EPPK, 241633B5, EPIPL, EPIPL1, Epiplakin

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Galerie de données de validation

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  WB analysis of L02 using 84344-2-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 84344-2-RR (EPPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84344-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EPPK1 antibody (84344-2-RR, Clone: 241633B5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of MCF-7 using 84344-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 84344-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84344-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84344-2-RR against Human EPPK1 were performed. The affinity constant is 1.61 nM.

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Applications testées

• Résultats positifs en WB: cellules L02,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules MCF-7, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84344-2-RR cible EPPK1 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPPK1 Protéine recombinante Ag35749
• Nom complet: epiplakin 1
• Masse moléculaire calculée: 555 kDa
• Poids moléculaire observé: 500-600 kDa
• Numéro d’acquisition GenBank: NM_031308.3
• Symbole du gène: EPPK1
• Identification du gène (NCBI): 83481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epplakin 1 (EPPK1), belonging to the plakin family, is involved in connecting intermediate filaments and regulating their reorganization in stress response. Studies have shown that EPPK1 is involved in the progression of various cancers, such as liver cancer, cervical cancer, and bladder urothelial carcinoma.

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