Anticorps Recombinant de lapin anti-EPS15

• Phare
• Validé par KD/KO

EPS15 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241485B7

N° de cat : 84179-1-PBS

Synonymes

241485B7, AF 1P, AF1P, MLLT5, Protein AF 1p

---

Galerie de données de validation

• 

  WB analysis using 84179-1-RR (same clone as 84179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84179-1-RR (EPS15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84179-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 84179-1-RR (same clone as 84179-1-PBS)

  WB result of EPS15 antibody (84179-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS15 transfected MCF-7 cells. This data was developed using the same antibody clone with 84179-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01095-1

  Cytometric bead array standard curve of MP01095-1, EPS15 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84179-1-PBS. Detection antibody: 84179-4-PBS. Standard: Ag35282. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84179-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84179-1-PBS against Human EPS15 was performed. The affinity constant is 3.07 nM.

• 

  Affinity and Kinetic Characterization of 84179-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84179-1-RR against Human EPS15 were performed. The affinity constant is 2.72 nM.

---

Informations sur le produit

84179-1-PBS cible EPS15 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPS15 Protéine recombinante Ag35282
• Nom complet: epidermal growth factor receptor pathway substrate 15
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_001981.3
• Symbole du gène: EPS15
• Identification du gène (NCBI): 2060
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EPS15 was originally identified as a substrate for the kinase activity of the epidermal growth factor receptor (EGFR). EPS15 has a tripartite structure comprising an NH2-terminal portion, which contains three EH domains, a central putative coiled-coil region, and a COOH-terminal domain containing multiple copies of the amino acid triplet Aspartate-Proline-Phenylalanine (PMID: 10481267). EPS15 is phosphorylated on tyrosine residues following EGFR activation (PMID: 7689153).

{{ptg:RelatedPrimaryAntibodies}}