Anticorps Polyclonal de lapin anti-EPS8

• Phare
• Validé par KD/KO

EPS8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12455-1-AP

Synonymes

Epidermal growth factor receptor kinase substrate 8

---

Galerie de données de validation

• 

  WB analysis using 12455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12455-1-AP (EPS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12455-1-AP

  WB result of EPS8 antibody (12455-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8 transfected HeLa cells.

• 

  IP experiment of HeLa using 12455-1-AP

  IP result of anti-EPS8 (IP:12455-1-AP, 3ug; Detection:12455-1-AP 1:500) with HeLa cells lysate 3800ug.

• 

  IHC staining of human breast cancer using 12455-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12455-1-AP (EPS8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12455-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12455-1-AP (EPS8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 12455-1-AP

  Immunofluorescent analysis of A375 cells using 12455-1-AP (EPS8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

12455-1-AP cible EPS8 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPS8 Protéine recombinante Ag3121
• Nom complet: epidermal growth factor receptor pathway substrate 8
• Masse moléculaire calculée: 822 aa, 92 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC030010
• Symbole du gène: EPS8
• Identification du gène (NCBI): 2059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.

Publications

Species	Application	Title
human	WB,IHC	Exp Cell Res; EPS8 supports pancreatic cancer growth by inhibiting BMI1 mediated proteasomal degradation of ALDH7A1.; Authors - Mingzhu Tan ; KD Validated
human	WB	Acta Biochim Biophys Sin (Shanghai); miR-345 inhibits migration and stem-like cell phenotype in gastric cancer via inactivation of Rac1 by targeting EPS8.; Authors - Jieyun Zhang ; KD Validated
mouse	WB	Acta Otolaryngol; The 133-kDa N-terminal region of myosin XVa is critical for normal structure and function of auditory and hair cells; Authors - Yong Li

{{ptg:RelatedPrimaryAntibodies}}