Anticorps Recombinant de lapin anti-EPS8

EPS8 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242835G12

N° de cat : 85184-5-PBS

Synonymes

Epidermal growth factor receptor kinase substrate 8

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Galerie de données de validation

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  WB analysis using 85184-5-RR (same clone as 85184-5-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85184-5-RR (EPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85184-5-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 85184-5-RR (same clone as 85184-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85184-5-RR (EPS8 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85184-5-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 85184-5-RR (same clone as 85184-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85184-5-RR (EPS8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85184-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85184-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85184-5-RR against Human EPS8 were performed. The affinity constant is 27.9 nM.

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Informations sur le produit

85184-5-PBS cible EPS8 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EPS8 Protéine recombinante Ag3121
• Nom complet: epidermal growth factor receptor pathway substrate 8
• Masse moléculaire calculée: 822 aa, 92 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC030010
• Symbole du gène: EPS8
• Identification du gène (NCBI): 2059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.

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