Anticorps Polyclonal de lapin anti-ER

ER Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 20698-1-AP

Synonymes

ESR1, ER alpha, Era, ER-alpha, ERα

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Galerie de données de validation

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  WB analysis using 20698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20698-1-AP (ER antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20698-1-AP (ER antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20698-1-AP (ER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20698-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20698-1-AP (ER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 20698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (20698-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

20698-1-AP cible ER dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: estrogen receptor 1
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: NM_000125
• Symbole du gène: ER
• Identification du gène (NCBI): 2099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Publications

Species	Application	Title
human	WB	Eur J Med Chem; Discovery of non-antiproliferative selective estrogen receptor degraders (SERDs) based on scaffold optimization of elacestrant; Authors - Yunlong Lu
mouse	WB	Cytotechnology; β-sitosterol in Yijing Hugui decoction prevents cyclophosphamide-induced premature ovarian insufficiency via the AKT1/Nrf2 pathway; Authors - Li Chen
mouse	IHC	Cell Death Dis; Metabolic shifts in lipid utilization and reciprocal interactions within the lung metastatic niche of triple-negative breast cancer revealed by spatial multi-omics; Authors - Jung-Yu Kan
human	WB	Exp Cell Res; EFHD1 promotes breast cancer progression through estrogen signaling; Authors - Xiaojun Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford. Z (Verified Customer) (01-31-2024)

Significant bands were observed across numerous cell lines, including instances where cells were not expected to exhibit such robust expression intensity. It raises the possibility that a monoclonal antibody might yield more precise results compared to the polyclonal antibody currently employed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines

ER Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:20698-1-AP)

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