Anticorps Polyclonal de lapin anti-ER

ER Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20698-1-PBS

Synonymes

ESR1, ER alpha, Era, ER-alpha, ERα

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Galerie de données de validation

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  WB analysis using 20698-1-AP (same clone as 20698-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20698-1-AP (ER antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20698-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20698-1-AP (same clone as 20698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20698-1-AP (ER antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20698-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20698-1-AP (same clone as 20698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20698-1-AP (ER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20698-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 20698-1-AP (same clone as 20698-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20698-1-AP (ER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20698-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 20698-1-AP (same clone as 20698-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (20698-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20698-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20698-1-PBS cible ER dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: estrogen receptor 1
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: NM_000125
• Symbole du gène: ER
• Identification du gène (NCBI): 2099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

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