Anticorps Monoclonal anti-ERAB

• Phare
• Validé par KD/KO

ERAB Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G5E5

N° de cat : 60069-1-PBS

Synonymes

HSD17B10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3alpha(or 20beta)-hydroxysteroid dehydrogenase, 3-alpha-(17-beta)-hydroxysteroid dehydrogenase, 2-methyl-3-hydroxybutyryl-CoA dehydrogenase

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 60069-1-Ig (same clone as 60069-1-PBS)

  WB result of ERAB antibody (60069-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells. This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 60069-1-Ig (same clone as 60069-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60069-1-Ig (same clone as 60069-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60069-1-Ig (same clone as 60069-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60069-1-Ig (same clone as 60069-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 60069-1-Ig (same clone as 60069-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60069-1-Ig (ERAB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60069-1-Ig (same clone as 60069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60069-1-Ig (same clone as 60069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60069-1-Ig (ERAB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60069-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50654-2

  Cytometric bead array standard curve of MP50654-2, HSD17B10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60069-1-PBS. Detection antibody: 60069-4-PBS. Standard:Ag1020. Range: 0.098-50 ng/mL.

---

Informations sur le produit

60069-1-PBS cible ERAB dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ERAB Protéine recombinante Ag1020
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 10
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC008708
• Symbole du gène: ERAB
• Identification du gène (NCBI): 3028
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

{{ptg:RelatedPrimaryAntibodies}}