Anticorps Polyclonal de lapin anti-ERAP2

ERAP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 14637-1-AP

Synonymes

ERAP2, L RAP, LRAP

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Galerie de données de validation

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  WB analysis of K-562 using 14637-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14637-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14637-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14637-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14637-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14637-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 14637-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14637-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A549, tissu de thymus de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14637-1-AP cible ERAP2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERAP2 Protéine recombinante Ag6216
• Nom complet: endoplasmic reticulum aminopeptidase 2
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 61-65 kDa
• Numéro d’acquisition GenBank: BC065240
• Symbole du gène: ERAP2
• Identification du gène (NCBI): 64167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase) and belongs to the peptidase M1 family. It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. It can form heterodimers with ERAP1(PMID:15908954). It has 4 isoforms produced by alternative splicing. And defects in the expression of this gene may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance.

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